5-Ethynyl-2'-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by "click" chemistry

被引：6
|
作者：
Chen MiaoJuan [1 ,2 ]
Qu DeZhong [1 ,2 ,4 ]
Chi WeiLin [1 ,2 ]
Wang Wei [1 ,2 ,4 ]
Ren XiaoShuai [1 ,2 ,5 ]
Cong ShuJie [1 ,2 ,4 ]
Liang PeiZhou [1 ,2 ,5 ]
Feng ShiPeng [6 ]
Zhang BiLiang [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Lab RNA Chem Biol, Guangzhou Inst Biomed, Guangzhou 510530, Guangdong, Peoples R China
[2] Chinese Acad Sci, Lab RNA Chem Biol, Guangzhou Inst Hlth, Guangzhou 510530, Guangdong, Peoples R China
[3] Guangzhou Inst Resp Dis, State Key Lab Resp Dis, Guangzhou 510182, Guangdong, Peoples R China
[4] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[5] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
[6] Guangzhou RiboBio Co Ltd, Guangzhou 510663, Guangdong, Peoples R China
来源：
SCIENCE CHINA-CHEMISTRY | 2011年 / 54卷 / 11期
基金：
中国国家自然科学基金;
关键词：
molecular probe; siRNA; miRNA; click" chemistry; FLOW-CYTOMETRY;
D O I：
10.1007/s11426-011-4398-4
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent alternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
引用
收藏
页码：1702 / 1710
页数：9
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