Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein

被引：104

作者：

Fukushi, S

Mizutani, T

Saijo, M

Matsuyama, S

Miyajima, N

Taguchi, F

Itamura, S

Kurane, I

Morikawa, S

机构：

[1] Natl Inst Infect Dis, Special Pathogens Lab, Dept Virol 1, Lab Resp Viral Dis, Tokyo 2080011, Japan

[2] Natl Inst Infect Dis, SARS, Tokyo 2080011, Japan

[3] Natl Inst Infect Dis, Lab Influenza Virus, Dept Virol 3, Tokyo 2080011, Japan

来源：

JOURNAL OF GENERAL VIROLOGY | 2005年 / 86卷

关键词：

D O I：

10.1099/vir.0.80955-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSV Delta G* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 In after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.

引用

页码：2269 / 2274

页数：6

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