Influence of Tip Size on Single Yeast Cell Imaging Using Scanning Electrochemical Microscopy

被引：19

作者：

Nagamine, Kuniaki ^{[1
]}

Takahashi, Yasufumi ^{[1
]}

Ino, Kosuke ^{[1
]}

Shiku, Hitoshi ^{[1
]}

Matsue, Tomokazu ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, Sendai, Miyagi 9808579, Japan

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 05期

关键词：

Scanning electrochemical microscopy; Nanoelectrodes; Single cell analysis; CONE-SHAPED MICROWELL; LIVING CELLS; ALGAL PROTOPLAST; REDOX ACTIVITY; SACCHAROMYCES-CEREVISIAE; RESPIRATION ACTIVITY; OXYGEN; NANOELECTRODES; MEDIATORS; MENADIONE;

D O I：

10.1002/elan.201000595

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in mu m-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

引用

页码：1168 / 1174

页数：7

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