Influence of Tip Size on Single Yeast Cell Imaging Using Scanning Electrochemical Microscopy

被引：19
|
作者：
Nagamine, Kuniaki [1 ]
Takahashi, Yasufumi [1 ]
Ino, Kosuke [1 ]
Shiku, Hitoshi [1 ]
Matsue, Tomokazu [1 ]
机构：
[1] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, Sendai, Miyagi 9808579, Japan
来源：
ELECTROANALYSIS | 2011年 / 23卷 / 05期
关键词：
Scanning electrochemical microscopy; Nanoelectrodes; Single cell analysis; CONE-SHAPED MICROWELL; LIVING CELLS; ALGAL PROTOPLAST; REDOX ACTIVITY; SACCHAROMYCES-CEREVISIAE; RESPIRATION ACTIVITY; OXYGEN; NANOELECTRODES; MEDIATORS; MENADIONE;
D O I：
10.1002/elan.201000595
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in mu m-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.
引用
收藏
页码：1168 / 1174
页数：7
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