Inhibitory effect of CT domain of CCN3/NOV on proliferation and differentiation of osteogenic mesenchymal stem cells, Kusa-A1

被引：35
|
作者：
Katsuki, Yuko [1 ,2 ]
Sakamoto, Kel [1 ]
Minamizato, Tokutaro [1 ]
Makino, Hatsune
Umezawa, Akihiro [3 ]
Ikeda, Masa-Aki [1 ]
Perbal, Bernard [4 ]
Amagasa, Teruo [2 ]
Yamaguchi, Akira
Katsube, Ken-Ichi [1 ]
机构：
[1] Tokyo Med & Dent Univ, Grad Sch, Bunkyo Ku, Tokyo 1138549, Japan
[2] Tokyo Med & Dent Univ, Grad Sch, Tokyo, Japan
[3] NICHHD, Dept Reprod Biol, Tokyo, Japan
[4] Univ Paris 7 D Diderot, Lab Oncol Virale & Mol, Paris, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 368卷 / 03期
基金：
日本学术振兴会;
关键词：
CCN3; NOV; Notch; p21; osteogenesis; BMP;
D O I：
10.1016/j.bbrc.2008.02.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
CCN3/NOV activates the Notch signal through the carboxyl terminal cysteine-rich (CT) domain. CCN3 transfection to Kusa-A1 inhibited osteogenic differentiation and cell proliferation, which is accompanied by upregulation of Hes/Hey, Notch downstream targets, and p21, a CDK inhibitor. Upregulation of Hes/Hey and p21 was abrogated by the deletion of CT domain. Anti-proliferative activity of CCN3 was also abrogated by CT domain deletion whereas anti-osteogenic activity was not completely abrogated. We found that CT domain-deleted CCN3 still possesses antagonistic effect on BMP-2. These results suggest that CCN3 employs Notch and BMP pathways in anti-osteogenic activity while it inhibits cell proliferation uniquely by Notch/p21 pathway. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：808 / 814
页数：7
相关论文
共 50 条
  • [1] The Role of CCN3 in Mesenchymal Stem Cells
    Katsube, Ken-ichi
    CCN PROTEINS IN HEALTH AND DISEASE: AN OVERVIEW OF THE FIFTH INTERNATIONAL WORKSHOP ON THE CCN FAMILY OF GENES, 2010, : 111 - 119
  • [2] CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway
    Luan, Yan
    Zhang, Hanyue
    Ma, Kaige
    Liu, Yingfei
    Lu, Haixia
    Chen, Xinlin
    Liu, Yong
    Zhang, Zhichao
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (12)
  • [3] Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1
    Pang, Shen
    Shen, Jia
    Liu, Yi
    Chen, Feng
    Zheng, Zhong
    James, Aaron W.
    Hsu, Ching-Yun
    Zhang, Hong
    Lee, Kevin S.
    Wang, Chunling
    Li, Chenshuang
    Chen, Xuepeng
    Jia, Haichao
    Zhang, Xinli
    Soo, Chia
    Ting, Kang
    STEM CELLS, 2015, 33 (03) : 904 - 915
  • [4] Caveolin-1 regulates proliferation and osteogenic differentiation of human mesenchymal stem cells
    Baker, Natasha
    Zhang, Guofeng
    You, Yang
    Tuan, Rocky S.
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2012, 113 (12) : 3773 - 3787
  • [5] Inhibitory effect of alcohol on osteogenic differentiation in human bone marrow-derived mesenchymal stem cells
    Gong, ZD
    Wezeman, FH
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2004, 28 (03) : 468 - 479
  • [6] Human amnion mesenchymal stem cells promote proliferation and osteogenic differentiation in human bone marrow mesenchymal stem cells
    Yuli Wang
    Ying Yin
    Fei Jiang
    Ning Chen
    Journal of Molecular Histology, 2015, 46 : 13 - 20
  • [7] Human amnion mesenchymal stem cells promote proliferation and osteogenic differentiation in human bone marrow mesenchymal stem cells
    Wang, Yuli
    Yin, Ying
    Jiang, Fei
    Chen, Ning
    JOURNAL OF MOLECULAR HISTOLOGY, 2015, 46 (01) : 13 - 20
  • [8] Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells
    Boland, GM
    Perkins, G
    Hall, DJ
    Tuan, RS
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2004, 93 (06) : 1210 - 1230
  • [9] THE EFFECT OF AUXETIC METAMATERIAL SCAFFOLDS IN OSTEOGENIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS
    Flamourakis, George
    Manganas, Phanee
    Bamparis, Georgios
    Farsari, Maria
    Ranella, Anthi
    TISSUE ENGINEERING PART A, 2023, 29 (11-12) : 354 - 354
  • [10] CCN3 and DLL1 co-regulate osteogenic differentiation of mouse embryonic fibroblasts in a Hey1-dependent manner
    Su, Xin
    Wei, Yalin
    Cao, Junjie
    Wu, Xiulin
    Mou, Daiyong
    Luo, Jinyong
    Li, Aifang
    Zuo, Guo-Wei
    Tang, Min
    CELL DEATH & DISEASE, 2018, 9
12345