Imaging skeletal pathology in mutant mice by microcomputed tomography

Objective. We describe the utility of microcomputed tomography (muCT) for imaging skeletal abnormalities in rodent model systems. For the purpose of illustration, the progressive ankylosis (ank) mutant was selected. ank mice develop prominent articular and periarticular calcifications at multiple anatomical sites, including paws, elbows, knees, and vertebrae. Methods. Forelimbs, hindlimbs, and proximal tail vertebrae of 4-month-old female ank/ank mice were scanned at 15 mum resolution using a SkyScan 1072 muCT instrument and images were generated using Analyze 4.0 software. Results. This technique was able to show, in 3-dimensional images, the abnormal calcification of ank/ank mice, which was readily observed within joint surfaces, on periosteal surfaces, sesamoid bones, menisci, and joint capsules, as well as other periarticular ligamentous structures. Conclusion. As illustrated by the example of the progressive ankylosis mutant, pCT represents a powerful emerging tool for identifying and monitoring the progression of developmental or acquired skeletal abnormalities within rodent models.