MODIFICATION OF GENE EXPRESSION IN MESENCHYMAL STROMAL CELLS OF THE ACUTE MYELOID LEUKEMIA PATIENTS DURING CHEMOTHERAPY

Aim of the study. To investigate the relevant expression level in multipotent mesenchymal stromal cells (MMCs) derived from the bone marrow (BM) of acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) patients before and over the course of chemotherapy. Material and Methods. BM derived MMSCs from 33 AML patients and 21 ALL patients were studied before and during chemotherapy. Total RNA was extracted from the MMSCs and the cDNA was synthesized. Gene expression levels were quantified by real-time quantitative PCR (RT-qPCR) with the use of gene-specific primers. Results. Before chemotherapy, the analysis of the gene expression of MMSCs from acute leukemia patients revealed a significant increase in the relative expression level (REL) of genes (IL-6, IL-8, IL-1b, CSF, JAG1, ICAM, VCAM) which regulate leukemic cell proliferation and migration. The REL of genes regulating MMSC proliferation and differentiation (IL-1R1, PDGERa, IGF, FGFR1, FGFR2, BGLAP) increased during chemotherapy. The alterations of bone marrow stroma were more pronounced in patients who didn't achieve remission.