Stretched and overwound DNA forms a Pauling-like structure with exposed bases

被引：284
作者：
Allemand, JF
Bensimon, D
Lavery, R
Croquette, V
机构：
[1] Univ Paris 06, Ecole Normale Super, Lab Phys Stat, URA D 1306 CNRS, F-75231 Paris 05, France
[2] Univ Paris 07, F-75231 Paris, France
[3] CNRS, Inst Biol Physicochim, Lab Biochim Theor, Unite Propre Rech 9080, F-75005 Paris, France
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 24期
关键词：
D O I：
10.1073/pnas.95.24.14152
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We investigate structural transitions within a single stretched and supercoiled DNA molecule. With negative supercoiling, for a stretching force >0.3 pN, we observe the coexistence of B-DNA and denatured DNA from sigma approximate to -0.015 down to sigma = -1. Surprisingly, for positively supercoiled DNA (sigma > +0.037) stretched by 3 pN, we observe a similar coexistence of B-DNA and a new highly twisted structure. Experimental data and molecular modeling suggest that this structure has approximate to 2.62 bases per turn and an extension 75% larger than B-DNA. This structure has tightly interwound phosphate backbones and exposed bases in common with Pauling's early DNA structure [Pauling, L. & Corey, R. B. (1953), Proc. Natl. Acad. Sci. USA 39, 84-97] and an unusual structure proposed for the Pf1 bacteriophage
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页码：14152 / 14157
页数：6
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