Fluorescence Quenching, Lifetimes, and Fluorophore Solvent Accessibility

被引：5
|
作者：
Stella, Lorenzo [1 ]
机构：
[1] Univ Roma Tor Vergata, Dept Chem Sci & Technol, I-00133 Rome, Italy
来源：
JOURNAL OF CHEMICAL EDUCATION | 2011年 / 88卷 / 06期
关键词：
Upper-Division Undergraduate; Physical Chemistry; Misconceptions/Discrepant Events; Fluorescence Spectroscopy; Proteins/Peptides; PHYSICAL-CHEMISTRY STUDENTS; CYTOCHROME-C; PROTEINS; VISCOSITY;
D O I：
10.1021/ed101147s
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The influence of the excited-state lifetime of a fluorophore on its susceptibility to collisional quenching is discussed. In the case of cytochrome c, FRET to the heme moiety strongly reduces the tryptophan fluorescence lifetime. This should be taken into account to correctly evaluate the tryptophan solvent accessibility from acrylamide quenching experiments.
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页码：695 / 696
页数：2
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