Fluorescence Quenching, Lifetimes, and Fluorophore Solvent Accessibility

被引:5
|
作者
Stella, Lorenzo [1 ]
机构
[1] Univ Roma Tor Vergata, Dept Chem Sci & Technol, I-00133 Rome, Italy
关键词
Upper-Division Undergraduate; Physical Chemistry; Misconceptions/Discrepant Events; Fluorescence Spectroscopy; Proteins/Peptides; PHYSICAL-CHEMISTRY STUDENTS; CYTOCHROME-C; PROTEINS; VISCOSITY;
D O I
10.1021/ed101147s
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The influence of the excited-state lifetime of a fluorophore on its susceptibility to collisional quenching is discussed. In the case of cytochrome c, FRET to the heme moiety strongly reduces the tryptophan fluorescence lifetime. This should be taken into account to correctly evaluate the tryptophan solvent accessibility from acrylamide quenching experiments.
引用
收藏
页码:695 / 696
页数:2
相关论文
共 50 条
  • [1] SOLVENT PERTURBATION OF THE FLUORESCENCE OF FLUORESCEIN BOUND TO SPECIFIC ANTIBODY - FLUORESCENCE QUENCHING OF THE BOUND FLUOROPHORE BY IODIDE
    WATT, RM
    VOSS, EW
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1979, 254 (05) : 1684 - 1690
  • [2] Effect of fluorescence quenching by stimulated emission on the spectral properties of a solvent-sensitive fluorophore
    Gryczynski, I
    Kusba, J
    Gryczynski, Z
    Malak, H
    Lakowicz, JR
    JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (24): : 10135 - 10144
  • [3] SOLVENT QUENCHING OF FLUORESCENCE
    BOWEN, EJ
    STEBBENS, DM
    JOURNAL OF THE CHEMICAL SOCIETY, 1957, (JAN): : 360 - 363
  • [4] Copper(II)-induced Fluorescence Quenching of a BODIPY Fluorophore
    Herten, Dirk-Peter
    Haderspeck, Andreas
    Braun, Felix
    Wadepohl, Hubert
    ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 2018, 644 (14): : 735 - 739
  • [5] SOLVENT QUENCHING FLUORESCENCE IMMUNOASSAY
    HALFMAN, CJ
    WONG, FCL
    CLINICAL CHEMISTRY, 1982, 28 (07) : 1657 - 1657
  • [6] SOLVENT QUENCHING OF THE FLUORESCENCE OF ANTHRACENE
    BOWEN, EJ
    WEST, K
    JOURNAL OF THE CHEMICAL SOCIETY, 1955, : 4394 - 4395
  • [7] Protein tryptophan accessibility studied by fluorescence quenching
    Möller, M
    Denicola, A
    BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, 2002, 30 (03) : 175 - 178
  • [8] Solvent dependence of the fluorescence lifetimes of xanthene dyes
    Magde, D
    Rojas, GE
    Seybold, PG
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1999, 70 (05) : 737 - 744
  • [9] FLUORESCENCE QUENCHING OF HUMAN OROSOMUCOID - ACCESSIBILITY TO DRUGS AND SMALL QUENCHING AGENTS
    FRIEDMAN, ML
    SCHLUETER, KT
    KIRLEY, TL
    HALSALL, HB
    BIOCHEMICAL JOURNAL, 1985, 232 (03) : 863 - 867
  • [10] Effect of solvent on fluorescence and fluorescence lifetimes of p-substituted polystyrene
    Yousef, Yaser A.
    Al-Ani, Khalid E.
    Akasheh, Talal
    ASIAN JOURNAL OF CHEMISTRY, 2006, 18 (03) : 1675 - 1684