Challenges in quantifying microbial gene expression in soil using quantitative reverse transcription real-time PCR

被引：24
|
作者：
Saleh-Lakha, Saleema [1 ]
Shannon, Kelly E. [1 ]
Goyer, Claudia [2 ]
Trevors, Jack T. [1 ]
机构：
[1] Univ Guelph, Microbiol Lab, Sch Environm Sci, Guelph, ON N1G 2W1, Canada
[2] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 85卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Environment; Expression; Gene; Nucleic acids; qRT-PCR; Soil; MESSENGER-RNA; DENITRIFICATION; QUANTIFICATION; CULTURABILITY; AMPLIFICATION; CULTIVATION; EXTRACTION; DIVERSITY; REDUCTASE; BACTERIA;
D O I：
10.1016/j.mimet.2011.03.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The quantification of microbial gene expression in diverse soil samples via quantitative reverse transcription real time polymerase chain reaction (gRT-PCR) has numerous challenges including total RNA extraction, sample preparation, gRT-PCR optimization and the correlation of gene expression with function. Despite these challenges, microbial gene expression has been successfully quantified in soil microorganisms, and will yield valuable information on soil functions and expression of genes in soil samples. In this perspective we discuss challenges of measuring microbial gene expression and highlight recent applications using gRT-PCR to research gene function in soil. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：239 / 243
页数：5
相关论文
共 50 条
  • [41] Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR
    Ding, Zanbo
    Cui, Jinghua
    Zhang, Qun
    Feng, Junxia
    Du, Bing
    Xue, Guanhua
    Yan, Chao
    Gan, Lin
    Fan, Zheng
    Feng, Yanling
    Zhao, Hanqing
    Xu, Ziying
    Yu, Zihui
    Fu, Tongtong
    Zhang, Rui
    Cui, Xiaohu
    Tian, Ziyan
    Chen, Jinfeng
    Chen, Yujie
    Li, Zhoufei
    Zhong, Xuemei
    Lin, Yanbing
    Yuan, Jing
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2024, 108 (01) : 21 - 21
  • [42] Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR
    Zanbo Ding
    Jinghua Cui
    Qun Zhang
    Junxia Feng
    Bing Du
    Guanhua Xue
    Chao Yan
    Lin Gan
    Zheng Fan
    Yanling Feng
    Hanqing Zhao
    Ziying Xu
    Zihui Yu
    Tongtong Fu
    Rui Zhang
    Xiaohu Cui
    Ziyan Tian
    Jinfeng Chen
    Yujie Chen
    Zhoufei Li
    Xuemei Zhong
    Yanbing Lin
    Jing Yuan
    Applied Microbiology and Biotechnology, 2024, 108
  • [43] Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    Bustin, SA
    JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 29 (01) : 23 - 39
  • [44] Real-time Reverse Transcription PCR Analysis for Validation of Transketolase Gene in Hepatocellular Carcinoma Tissues
    Kim, Jongmin
    Hong, Seok Joo
    Park, Jun Ho
    Lee, Chang-Bae
    Gu, HongDu
    Kim, Seung Whan
    Choi, Gyu-Seong
    Kwon, Choon Hyuck David
    Joh, Jae-Won
    Kim, Dae Shick
    BIOCHIP JOURNAL, 2009, 3 (02) : 130 - 138
  • [45] Gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time PCR
    Jutta Zagon
    Bärbel Jansen
    Meike Knoppik
    Anke Ehlers
    Lothar W. Kroh
    Thomas Holzhauser
    Stefan Vieths
    Hermann Broll
    Analytical and Bioanalytical Chemistry, 2010, 396 : 483 - 493
  • [46] Gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time PCR
    Zagon, Jutta
    Jansen, Baerbel
    Knoppik, Meike
    Ehlers, Anke
    Kroh, Lothar W.
    Holzhauser, Thomas
    Vieths, Stefan
    Broll, Hermann
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (01) : 483 - 493
  • [47] A versatile internal control for use as DNA in real-time PCR and as RNA in real-time reverse transcription PCR assays
    Deer, D. M.
    Lampel, K. A.
    Gonzalez-Escalona, N.
    LETTERS IN APPLIED MICROBIOLOGY, 2010, 50 (04) : 366 - 372
  • [48] Human disease characterization: real-time quantitative PCR analysis of gene expression
    Snider, JV
    Wechser, MA
    Lossos, IS
    DRUG DISCOVERY TODAY, 2001, 6 (20) : 1062 - 1067
  • [49] An overview of real-time quantitative PCR: Applications to quantify cytokine gene expression
    Giulietti, A
    Overbergh, L
    Valckx, D
    Decallonne, B
    Bouillon, R
    Mathieu, C
    METHODS, 2001, 25 (04) : 386 - 401
  • [50] Evaluation of Internal Control for Gene Expression in Phalaenopsis by Quantitative Real-Time PCR
    Yuan, Xiu-Yun
    Jiang, Su-Hua
    Wang, Mo-Fei
    Ma, Jie
    Zhang, Xian-Yun
    Cui, Bo
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2014, 173 (06) : 1431 - 1445
12345