Challenges in quantifying microbial gene expression in soil using quantitative reverse transcription real-time PCR

被引：24
|
作者：
Saleh-Lakha, Saleema [1 ]
Shannon, Kelly E. [1 ]
Goyer, Claudia [2 ]
Trevors, Jack T. [1 ]
机构：
[1] Univ Guelph, Microbiol Lab, Sch Environm Sci, Guelph, ON N1G 2W1, Canada
[2] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 85卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Environment; Expression; Gene; Nucleic acids; qRT-PCR; Soil; MESSENGER-RNA; DENITRIFICATION; QUANTIFICATION; CULTURABILITY; AMPLIFICATION; CULTIVATION; EXTRACTION; DIVERSITY; REDUCTASE; BACTERIA;
D O I：
10.1016/j.mimet.2011.03.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The quantification of microbial gene expression in diverse soil samples via quantitative reverse transcription real time polymerase chain reaction (gRT-PCR) has numerous challenges including total RNA extraction, sample preparation, gRT-PCR optimization and the correlation of gene expression with function. Despite these challenges, microbial gene expression has been successfully quantified in soil microorganisms, and will yield valuable information on soil functions and expression of genes in soil samples. In this perspective we discuss challenges of measuring microbial gene expression and highlight recent applications using gRT-PCR to research gene function in soil. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：239 / 243
页数：5
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