Syndecan-1 regulates dendritic cell migration in cutaneous hypersensitivity to haptens

被引：11

作者：

Averbeck, Marco ^{[1
]}

Kuhn, Stephanie ^{[2
]}

Buehligen, Johannes ^{[1
]}

Goette, Martin ^{[3
]}

Simon, Jan C. ^{[1
]}

Polte, Tobias ^{[1
,2
]}

机构：

[1] Univ Klinikum Leipzig, Dept Dermatol Venerol & Allergol, Leipzig, Germany

[2] UFZ Helmholtz Ctr Environm Res Leipzig Halle, Dept Environm Immunol, Leipzig, Germany

[3] Munster Univ Hosp, Dept Gynecol & Obstet, Munster, Germany

来源：

EXPERIMENTAL DERMATOLOGY | 2017年 / 26卷 / 11期

关键词：

cell migration; cutaneous hypersensitivity; dendritic cell; syndecan; HEPARAN-SULFATE; LANGERHANS CELLS; INTEGRIN; ADHESION; DISTINCT; GLYCOSAMINOGLYCANS; DEFICIENCY; EXPRESSION; MOTILITY;

D O I：

10.1111/exd.13374

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

In human dendritic cells (DCs), we previously demonstrated in vitro that syndecan-1 (SDC1) is downregulated during maturation correlating with enhanced motility. We investigated the effects of SDC1 on DC migration in vivo during TNCB(2,4,6-trinitro-1-chlorobenzene)-induced cutaneous hypersensitivity reaction (CHS) in mice. We show that DC in SDC1-deficient mice migrated faster and at a higher rate to lymph nodes draining the hapten-painted skin. Adoptive transfer of SDC1-deficient hapten- and fluorochrome-labelled DC into wild-type (WT) mice led to increased and faster migration of DC to paracortical lymph nodes, and to a stronger CHS compared to WT DC. In SDC1-/- mice, CCR7 remains longer on the DC surface within the first 15-minutes maturation (after LPS-induced maturation). In addition, a time-dependent upregulation of CCL2, CCL3, VCAM1 and talin was found during maturation in SDC1-/- DC. However, no difference in T-cell-stimulating capacity of SDC1-deficient DC was found compared to WT DC. Mechanistically, SDC1-deficient DC showed enhanced migration towards CCL21 and CCL19. This may result from functional overexpression of CCR7 in SDC1-/- DC. Increased and accelerated migration of otherwise functionally intact SDC1-deficient DC leads to an exacerbated CHS. Based on our results, we conclude that SDC1 on DC negatively regulates DC migration.

引用

页码：1060 / 1067

页数：8

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