Development, chromosome location and genetic mapping of EST-SSR markers in wheat

被引：28

作者：

Chen, HM ^{[1
]}

Li, LZ ^{[1
]}

Wei, XY ^{[1
]}

Li, SS ^{[1
]}

Lei, TD ^{[1
]}

Hu, HZ ^{[1
]}

Wang, HG ^{[1
]}

Zhang, XS ^{[1
]}

机构：

[1] Shandong Agr Univ, Shandong Key Lab Crop Biol, Coll Agron, Tai An 271018, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2005年 / 50卷 / 20期

基金：

中国国家自然科学基金;

关键词：

wheat; EST-SSR; primer; nulli-tetrasome; RIL; genetic map;

D O I：

10.1360/982005-379

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2-5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework map previously constructed using recombinant inbred lines.

引用

页码：2328 / 2336

页数：9

共 22 条

[1] A microsatellite map of white clover [J].

Barrett, B ;

Griffiths, A ;

Schreiber, M ;

Ellison, N ;

Mercer, C ;

Bouton, J ;

Ong, B ;

Forster, J ;

Sawbridge, T ;

Spangenberg, G ;

Bryan, G ;

Woodfield, D .

THEORETICAL AND APPLIED GENETICS, 2004, 109 (03) :596-608

[2] Maize simple repetitive DNA sequences: Abundance and allele variation [J].

Chin, ECL ;

Senior, ML ;

Shu, H ;

Smith, JSC .

GENOME, 1996, 39 (05) :866-873

[3] Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.) [J].

Cho, YG ;

Ishii, T ;

Temnykh, S ;

Chen, X ;

Lipovich, L ;

McCouch, SR ;

Park, WD ;

Ayres, N ;

Cartinhour, S .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (05) :713-722

[4] Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum [J].

Cordeiro, GM ;

Casu, R ;

McIntyre, CL ;

Manners, JM ;

Henry, RJ .

PLANT SCIENCE, 2001, 160 (06) :1115-1123

[5] Development and transferability of apricot and grape EST microsatellite markers across taxa [J].

Decroocq, V ;

Favé, MG ;

Hagen, L ;

Bordenave, L ;

Decroocq, S .

THEORETICAL AND APPLIED GENETICS, 2003, 106 (05) :912-922

[6] Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat [J].

Eujayl, I ;

Sorrells, ME ;

Baum, M ;

Wolters, P ;

Powell, W .

THEORETICAL AND APPLIED GENETICS, 2002, 104 (2-3) :399-407

[7] Analysis of microsatellites in major crops assessed by computational and experimental approaches [J].

Gao, LF ;

Tang, JF ;

Li, HW ;

Jia, JZ .

MOLECULAR BREEDING, 2003, 12 (03) :245-261

[8] One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat [J].

Gao, LF ;

Jing, RL ;

Huo, NX ;

Li, Y ;

Li, XP ;

Zhou, RH ;

Chang, XP ;

Tang, JF ;

Ma, ZY ;

Jia, JZ .

THEORETICAL AND APPLIED GENETICS, 2004, 108 (07) :1392-1400

[9] Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat [J].

Gupta, PK ;

Rustgi, S ;

Sharma, S ;

Singh, R ;

Kumar, N ;

Balyan, HS .

MOLECULAR GENETICS AND GENOMICS, 2003, 270 (04) :315-323

[10] Identification of microsatellite polymorphisms in an expressed portion of the rye genome [J].

Hackauf, B ;

Wehling, P .

PLANT BREEDING, 2002, 121 (01) :17-25

← 1 2 3 →