Differential regulation of brain-derived neurotrophic factor (BDNF) expression in sensory neuron axons by miRNA-206

被引：10

作者：

Shrestha, Shiva ^{[1
,2
,3
]}

Phay, Monichan ^{[1
,2
]}

Kim, Hak Hee ^{[1
]}

Pouladvand, Pedram ^{[2
,4
]}

Lee, Seung Joon ^{[5
]}

Yoo, Soonmoon ^{[1
,2
]}

机构：

[1] Alfred I duPont Hosp Children, Nemours Biomed Res, 1600 Rockland Rd, Wilmington, DE 19803 USA

[2] Univ Delaware, Dept Biol Sci, Newark, DE USA

[3] Univ Penn, Perelman Sch Med, Gene Therapy Program, Philadelphia, PA 19104 USA

[4] Penn State Univ Brandywine, Dept Biol, Media, PA USA

[5] Univ South Carolina, Dept Biol Sci, Columbia, SC USA

来源：

FEBS OPEN BIO | 2019年 / 9卷 / 02期

关键词：

3UTR variants; BDNF mRNA; brain-derived neurotrophic factor; differential regulation; dorsal root ganglion; miRNA-206; mRNA localization; sensory neuron; small non-coding microRNA; NEURITIN/CPG15; MESSENGER-RNA; INTRAAXONAL TRANSLATION; ALZHEIMERS-DISEASE; MICRORNA-206; LOCALIZATION; PROTEIN; POLYADENYLATION; SEQUENCES;

D O I：

10.1002/2211-5463.12581

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Distinct subcellular localization and subsequent translational control of 3UTR variants of mRNA encoding brain-derived neurotrophic factor (BDNF) are critical for the development and plasticity of neurons. Although the processes that lead to preferential localization of BDNF have been well studied, it is still not clear how neurons ensure differential BDNF production in a spatial-specific manner. Here, we identified that microRNA (miRNA)-206 has the potential to specifically regulate BDNF with a long 3UTR without affecting its short 3UTR counterpart. Overexpression of miRNA-206 in sensory neurons resulted in a 30% and 45% reduction of BDNF protein expression in the cell bodies and axons, respectively. The work described in the present study indicates that miRNAs can differentially and specifically regulate the expression of transcript variants with different localization patterns.

引用

页码：374 / 383

页数：10

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