Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells

被引：0

作者：

Sone, Yasuko ^{[1
]}

Ueta, Etsuko ^{[2
]}

Sannomaru, Yasuko ^{[3
]}

Miyake, Noriko ^{[4
]}

Sone, Hirohito ^{[5
]}

Otsuka, Yuzuru ^{[6
]}

Kondo, Kazuo ^{[6
]}

Kurata, Tadao ^{[7
]}

Suzuki, Emiko ^{[6
]}

机构：

[1] Ochanomizu Univ, Inst Environm Sci Human Life, Bunkyo Ku, Tokyo 1128610, Japan

[2] Tottori Univ, Fac Med, Yonago, Tottori 6838503, Japan

[3] Josai Univ, Fac Pharmaceut Sci, Sakado, Saitama 3500295, Japan

[4] Tokyo Kasei Gakuin Univ, Dept Modern Home Econ, Chiyoda Ku, Tokyo 102834, Japan

[5] Univ Tsukuba, Inst Clin Med, Dept Internal Med, Mito, Ibaraki 3100015, Japan

[6] Ochanomizu Univ, Grad Sch Humanities & Sci, Bunkyo Ku, Tokyo 1128610, Japan

[7] Kanagawa Inst Technol, Atsugi Kanagawa 2430292, Japan

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2011年 / 25卷 / 06期

基金：

日本学术振兴会;

关键词：

HepG2; l-ascorbic acid; 3-methylcholanthrene; ODS rat; Sodium-dependent vitamin C transporter; L-ASCORBIC-ACID; GENE-EXPRESSION; 2-O-ALPHA-D-GLUCOPYRANOSYL-L-ASCORBIC ACID; CYTOCHROME P4501A1; XENOBIOTICS; GLUCOSE; BLOOD; PHARMACOKINETICS; METABOLISM; TOXICITY;

D O I：

10.1002/jbt.20403

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polycyclic aromatic hydrocarbon (PAH) compounds including 3-methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3-methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l-ascorbic acid daily and intraperitoneal injections of 10 mg 3-methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium-dependent vitamin C transporter (SVCTs) 1 and the l-ascorbic acid concentration in 3-methylcholanthrene-treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3-methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics-metabolizing, such as the induction of cytochrome P450 family. (c) 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:369376 2011; View this article online at . DOI 10.1002/jbt.20403

引用

页码：369 / 376

页数：8

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