Identifying Diagnostic and Prognostic Biomarkers and Candidate Therapeutic Drugs of Gastric Cancer Based on Transcriptomics and Single-Cell Sequencing

被引:15
作者
Zhao, Xu [1 ]
Wu, Shuang [2 ]
Jing, Jingjing [3 ,4 ,5 ]
机构
[1] Liaoning Vocat Coll Med, Math Comp Teaching & Res Off, Shenyang, Peoples R China
[2] Changchun Normal Univ, Coll Comp Sci & Technol, Changchun, Peoples R China
[3] China Med Univ, Hosp 1, Tumor Etiol & Screening Dept, Canc Inst & Gen Surg, Shenyang, Peoples R China
[4] China Med Univ, Hosp 1, Key Lab Canc Etiol & Prevent, Liaoning Educ Dept, Shenyang, Peoples R China
[5] China Med Univ, Hosp 1, Key Lab GI Canc Etiol & Prevent Liaoning Prov, Shenyang, Peoples R China
基金
中国国家自然科学基金;
关键词
bioinformatics; biomarkers; prognosis; gastric cancer; hub genes; molecular drugs; SPARC EXPRESSION; VEGF EXPRESSION; POOR-PROGNOSIS; WEB SERVER; BIGLYCAN; PROGRESSION; INHIBITION; GENES;
D O I
10.3389/pore.2021.1609955
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background and Objective: Gastric cancer (GC) is an important health burden and the prognosis of GC is poor. We aimed to explore new diagnostic and prognostic indicators as well as potential therapeutic targets for GC in the current study.Methods: We screened the overlapped differentially expressed genes (DEGs) from GSE54129 and TCGA STAD datasets. Protein-protein interaction network analysis recognized the hub genes among the DEGs. The roles of these genes in diagnosis, prognosis, and their relationship with immune infiltrates and drug sensitivity of GC were analyzed using R studio. Finally, the clinically significant hub genes were verified using single-cell RNA sequencing (scRNA-seq) data.Results: A total of 222 overlapping genes were screened, which were enriched in extracellular matrix-related pathways. Further, 17 hub genes were identified, and our findings demonstrated that BGN, COMP, COL5A2, and SPARC might be important diagnostic and prognostic indicators of GC, which were also correlated with immune cell infiltration, tumor mutation burden (TMB), microsatellite instability (MSI), and sensitivity of therapeutic drugs. The scRNA-seq results further confirmed that all four hub genes were highly expressed in GC.Conclusion: Based on transcriptomics and single-cell sequencing, we identified four diagnostic and prognostic biomarkers of GC, including BGN, COMP, COL5A2, and SPARC, which can help predict drug sensitivity for GC as well.
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页数:14
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