From DNA sequence analysis to modeling replication in the human genome

被引:40
作者
Brodie, EB [1 ]
Nicolay, S
Touchon, M
Audit, B
d'Aubenton-Carafa, Y
Thermes, C
Arneodo, A
机构
[1] Ecole Normale Super Lyon, CNRS, Lab Joliot Curie, 46 Allee Italie, F-69364 Lyon, France
[2] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France
关键词
D O I
10.1103/PhysRevLett.94.248103
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We explore the large-scale behavior of nucleotide compositional strand asymmetries along human chromosomes. As we observe for 7 of 9 origins of replication experimentally identified so far, the (TA+GC) skew displays rather sharp upward jumps, with a linear decreasing profile in between two successive jumps. We present a model of replication with well positioned replication origins and random terminations that accounts for the observed characteristic serrated skew profiles. We succeed in identifying 287 pairs of putative adjacent replication origins with an origin spacing similar to 1-2 Mbp that are likely to correspond to replication foci observed in interphase nuclei and recognized as stable structures that persist throughout subsequent cell generations.
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页数:4
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