Characterization of coplanar electrode structures for microfluidic-based impedance spectroscopy

Impedance spectroscopy has the potential for label-free integrated electrochemical detection in microfluidic lab-on-a-chip applications. Its capability to identify and discern between surface and bulk processes in solid-liquid systems finds particular use for the detection of biorecognition events or conductivity measurements. The electrochemical transducer can be in the form of interdigitated electrode structures to increase sensitivity. Experimental work was performed to characterize two different transducer designs. Applications included the monitoring of protein films on contact-less interdigitated electrode structures and conductivity detection of droplets on insulated two-electrode structures. The use of electrode passivation eliminated electrode degradation. Experimental results were compared to theoretical analytical models, and were found to closely correlate with one another. The analytical models were used to design the transducer for optimal conductivity detection. The results inform the current research efforts for the development of in-line impedance spectroscopy in digital microfluidics and confirm the use of simple analytical models for the first-order estimation of the frequency response of interdigitated electrode structures. (C) 2015 Elsevier B.V. All rights reserved.