Role of Elongator Subunit Elp3 in Drosophila melanogaster Larval Development and Immunity

被引：47

作者：

Walker, Jane ^{[1
]}

Kwon, So Yeon ^{[2
]}

Badenhorst, Paul ^{[2
]}

East, Phil ^{[3
]}

McNeill, Helen ^{[3
]}

Svejstrup, Jesper Q. ^{[1
]}

机构：

[1] Canc Res UK London Res Inst, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

[2] Univ Birmingham, Inst Biomed Res, Edgbaston B15 2TT, England

[3] Canc Res UK London Res Inst, Lincolns Inn Fields Labs, London WC2A 3PX, England

来源：

GENETICS | 2011年 / 187卷 / 04期

关键词：

POLYMERASE-II HOLOENZYME; HUMORAL STRESS-RESPONSE; REMODELING FACTOR NURF; IN-VIVO; TRANSCRIPTIONAL ELONGATION; HISTONE ACETYLTRANSFERASE; FAMILIAL DYSAUTONOMIA; GENE-EXPRESSION; CELL-MIGRATION; BROAD-COMPLEX;

D O I：

10.1534/genetics.110.123893

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for similar to 24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino.

引用

页码：1067 / U157

页数：12

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