Metabolic profiling of a transgenic Caenorhabditis elegans Alzheimer model

被引：24

作者：

Van Assche, Roel ^{[1
]}

Temmerman, Liesbet ^{[1
]}

Dias, Daniel A. ^{[2
]}

Boughton, Berin ^{[2
]}

Boonen, Kurt ^{[1
]}

Braeckman, Bart P. ^{[3
]}

Schoofs, Liliane ^{[1
]}

Roessner, Ute ^{[2
]}

机构：

[1] Katholieke Univ Leuven, Dept Biol, Funct Genom & Prote, Leuven, Belgium

[2] Univ Melbourne, Sch Bot, Metabol Australia, Melbourne, Vic, Australia

[3] Univ Ghent, Dept Biol, Lab Aging Physiol & Mol Evolut, B-9000 Ghent, Belgium

来源：

METABOLOMICS | 2015年 / 11卷 / 02期

关键词：

Metabolomics; Metabolic profiling; Caenorhabditis elegans; Alzheimer's disease; Amyloid-beta; BETA-AMYLOID PEPTIDE; OXIDATIVE STRESS; ALLANTOIN LEVELS; MOUSE MODEL; DISEASE; IDENTIFICATION; EXTRACTION; EXPRESSION; TAU;

D O I：

10.1007/s11306-014-0711-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Despite decades of research, no early-onset biomarkers are currently available for Alzheimer's disease, a cureless neurodegenerative disease afflicting millions worldwide. In this study, transgenic Caenorhabditis elegans were used to investigate changes in the metabolome after induced expression of amyloid-beta. GC- and LC-MS-based platforms determined a total of 157 differential features. Some of these were identified using in-house (GC-MS) or public libraries (LC-MS), revealing changes in allantoin, cystathionine and tyrosine levels. Since C. elegans is far better suited to metabolomics studies than most other model systems, the accordance of these findings with vertebrate literature is promising and argues for further use of C. elegans as a model of human pathology in the study of AD.

引用

页码：477 / 486

页数：10

共 50 条

[1] Association analysis of CβS 844ins68 and MTHFD1 G1958A polymorphisms with Alzheimer's disease in Chinese [J].

Bi, Xiu-Hua ;

Zhao, Hua-Lu ;

Zhang, Zhen-Xin ;

Liu, Qian ;

Zhang, Jun-Wu .

JOURNAL OF NEURAL TRANSMISSION, 2010, 117 (04) :499-503

[2] Fluid Biomarkers in Alzheimer Disease [J].

Blennow, Kaj ;

Zetterberg, Henrik ;

Fagan, Anne M. .

COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2012, 2 (09)

[3] Proteomic identification of proteins specifically oxidized in Caenorhabditis elegans expressing human Aβ(1-42):: Implications for Alzheimer's disease [J].

Boyd-Kimball, Debra ;

Poon, H. Fai ;

Lynn, Bert C. ;

Cai, Jian ;

Pierce, William M., Jr. ;

Klein, Jon B. ;

Ferguson, Jmil ;

Link, Christopher D. ;

Butterfield, D. Allan .

NEUROBIOLOGY OF AGING, 2006, 27 (09) :1239-1249

[4]

BRENNER S, 1974, GENETICS, V77, P71

[5]

De Livera AM, 2013, METHODS MOL BIOL, V1055, P291, DOI 10.1007/978-1-62703-577-4_20

[6]

De Vos Ann, 2011, Int J Alzheimers Dis, V2011, P428970, DOI 10.4061/2011/428970

[7] LC-MS Proteomics Analysis of the Insulin/IGF-1-Deficient Caenorhabditis elegans daf-2(e1370) Mutant Reveals Extensive Restructuring of Intermediary Metabolism [J].

Depuydt, Geert ;

Xie, Fang ;

Petyuk, Vladislav A. ;

Smolders, Arne ;

Brewer, Heather M. ;

Camp, David G., II ;

Smith, Richard D. ;

Braeckman, Bart P. .

JOURNAL OF PROTEOME RESEARCH, 2014, 13 (04) :1938-1956

[8] Behavioral Phenotyping of a Transgenic Caenorhabditis elegans Expressing Neuronal Amyloid-β [J].

Dosanjh, Laura E. ;

Brown, Marishka K. ;

Rao, Gautam ;

Link, Christopher D. ;

Luo, Yuan .

JOURNAL OF ALZHEIMERS DISEASE, 2010, 19 (02) :681-690

[9]

Dostal V, 2010, JOVE-J VIS EXP, V44, DOI [10.3791/2252, DOI 10.3791/2252]

[10] Transgenic Mouse Models of Alzheimer's Disease [J].

Elder, Gregory A. ;

Sosa, Miguel A. Gama ;

De Gasperi, Rita .

MOUNT SINAI JOURNAL OF MEDICINE, 2010, 77 (01) :69-81

← 1 2 3 4 5 →