Higher-order structure of barley chromosomes observed by electron tomography

被引：2

作者：

Hayashida, Misa ^{[1
]}

Sartsanga, Channarong ^{[2
]}

Phengchat, Rinyaporn ^{[2
]}

Malac, Marek ^{[1
,3
]}

Harada, Ken ^{[4
]}

Akashi, Tetsuya ^{[5
]}

Fukui, Kiichi ^{[6
]}

Ohmido, Nobuko ^{[2
]}

机构：

[1] Nanotechnol Res Ctr, Natl Res Council, 11421 Saskatchewan Dr, Edmonton, AB T6G 2M9, Canada

[2] Kobe Univ, Grad Sch Human Dev & Environm, Tsurukabuto 3-11,Nada ku, Kobe, 6578501, Japan

[3] Univ Alberta, Dept Phys, Edmonton, AB T6G 2E1, Canada

[4] RIKEN, Ctr Emergent Matter Sci CEMS, Hatoyama, Saitama 3500395, Japan

[5] Hitachi Ltd, Res & Dev Grp, Hatoyama, Saitama 3500395, Japan

[6] Osaka Univ, Grad Sch Pharmaceut Sci, Yamadaoka 1-6,Suita, Osaka 5650871, Japan

来源：

MICRON | 2022年 / 160卷

基金：

日本科学技术振兴机构;

关键词：

Electron tomography; Barley chromosomes; TEM; MICROSCOPY; ORGANIZATION; FIBERS;

D O I：

10.1016/j.micron.2022.103328

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The higher order structure of the metaphase chromosome has been an enigma for over a century and several different models have been presented based on results obtained by a variety of techniques. Some disagreements in the results between methods have possibly arisen from artifacts caused during sample preparation such as staining and dehydration. Therefore, we treated barley chromosomes with ionic liquid to minimize the effects of dehydration. We also observed chromosomes on a film with holes to keep pristine chromosome structure from being flattened as seen when placed on a continuous support film. A chromosome placed over a hole in a thin carbon film was mounted on a tomography holder, and its structure was observed in three dimensions (3D) using electron tomography. We found that there are periodic structures with 300-400 nm pitch along the axis in barley chromosomes. The pitch sizes are larger than those observed in human chromosomes.

引用

页数：6

共 36 条

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