Transcriptomic and functional analysis of cucumber (Cucumis sativus L.) fruit phloem during early development

被引:29
作者
Sui, Xiaolei [1 ]
Nie, Jing [1 ]
Li, Xin [1 ]
Scanlon, Michael J. [2 ]
Zhang, Cankui [3 ]
Zheng, Yi [4 ]
Ma, Si [1 ]
Shan, Nan [1 ]
Fei, Zhangjun [4 ]
Turgeon, Robert [2 ]
Zhang, Zhenxian [1 ]
机构
[1] China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China
[2] Cornell Univ, Sch Integrat Plant Sci, Plant Biol Sect, Ithaca, NY 14853 USA
[3] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA
[4] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14853 USA
基金
美国国家科学基金会; 美国食品与农业研究所; 中国国家自然科学基金;
关键词
cucumber (Cucumis sativus L.) fruit; laser capture microdissection; RNA sequencing; external phloem; internal phloem; vascular bundle; transport; defense response; SEED DEVELOPMENT; MOSAIC-VIRUS; AMINO-ACIDS; TRANSPORT; METABOLISM; OCCLUSION; SINK; ACCUMULATION; QUESTIONS; SYNTHASE;
D O I
10.1111/tpj.14084
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The phloem of the Cucurbitaceae has long been a subject of interest due to its complex nature and the economic importance of the family. As in a limited number of other families, cucurbit phloem is bicollateral, i.e. with sieve tubes on both sides of the xylem. To date little is known about the specialized functions of the internal phloem (IP) and external phloem (EP). Here, a combination of microscopy, fluorescent dye transport analysis, micro-computed tomography, laser capture microdissection and RNA-sequencing (RNA-Seq) were used to study the functions of IP and EP in the vascular bundles (VBs) of cucumber fruit. There is one type of VB in the peduncle, but four in the fruit: peripheral (PeVB), main (MVB), carpel (CVB) and placental (PlVB). The VBs are bicollateral, except for the CVB and PlVB. Phloem mobile tracers and C-14 applied to leaves are transported primarily in the EP, and to a lesser extent in the IP. RNA-Seq data indicate preferential gene transcription in the IP related to differentiation/development, hormone transport, RNA or protein modification/processing/transport, and nitrogen compound metabolism and transport. The EP preferentially expresses genes for stimulus/stress, defense, ion transport and secondary metabolite biosynthesis. The MVB phloem is preferentially involved in photoassimilate transport, unloading and long-distance signaling, while the PeVB plays a more substantial role in morphogenesis and/or development and defense response. CVB and PlVB transcripts are biased toward development of reproductive organs. These findings provide an integrated view of the differentiated structure and function of the vascular tissue in cucumber fruit.
引用
收藏
页码:982 / 996
页数:15
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