P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

被引：536

作者：

Hubstenberger, Arnaud ^{[1
,2
]}

Courel, Maite ^{[1
]}

Benard, Marianne ^{[1
]}

Souquere, Sylvie ^{[3
]}

Ernoult-Lange, Michele ^{[1
]}

Chouaib, Racha ^{[1
,4
]}

Yi, Zhou ^{[1
]}

Morlot, Jean-Baptiste ^{[8
]}

Munier, Annie ^{[5
]}

Fradet, Magali ^{[6
]}

Daunesse, Maelle ^{[7
]}

Bertrand, Edouard ^{[4
]}

Pierron, Gerard ^{[3
]}

Mozziconacci, Julien ^{[8
]}

Kress, Michel ^{[1
]}

Weil, Dominique ^{[1
]}

机构：

[1] UPMC Univ Paris 06, IBPS, CNRS, UMR 7622, F-75005 Paris, France

[2] Univ Cote Azur, CNRS, INSERM, iBV, Nice, France

[3] CNRS, UMR 9196, Inst Gustave Roussy, F-94800 Villejuif, France

[4] Univ Montpellier, CNRS, IGMM, F-34090 Montpellier, France

[5] UPMC Univ Paris 06, LUMIC, UMS30, F-75005 Paris, France

[6] UPMC Univ Paris 06, ICAN, F-75013 Paris, France

[7] PSL Res Univ, Ecole Normale Super, CNRS, INSERM,IBENS,Genom Paris Ctr, F-75005 Paris, France

[8] UPMC Univ Paris 06, LPTMC, F-75005 Paris, France

来源：

MOLECULAR CELL | 2017年 / 68卷 / 01期

关键词：

TRANSLATIONAL REPRESSION; STRESS GRANULES; PHASE-TRANSITIONS; TERNARY COMPLEX; GENE-EXPRESSION; BODIES; DECAY; PROTEIN; INITIATION; DYNAMICS;

D O I：

10.1016/j.molcel.2017.09.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within cells, soluble RNPs can switch states to coassemble and condense into liquid or solid bodies. Although these phase transitions have been reconstituted in vitro, for endogenous bodies the diversity of the components, the specificity of the interaction networks, and the function of the coassemblies remain to be characterized. Here, by developing a fluorescence-activated particle sorting (FAPS) method to purify cytosolic processing bodies (P-bodies) from human epithelial cells, we identified hundreds of proteins and thousands of mRNAs that structure a dense network of interactions, separating P-body from non-P-body RNPs. mRNAs segregating into P-bodies are translationally repressed, but not decayed, and this repression explains part of the poor genome-wide correlation between RNA and protein abundance. P-bodies condense thousands of mRNAs that strikingly encode regulatory processes. Thus, we uncovered how P-bodies, by condensing and segregating repressed mRNAs, provide a physical substrate for the coordinated regulation of posttranscriptional mRNA regulons.

引用

页码：144 / +

页数：19

共 55 条

[1] Quantifying mRNA targeting to P-bodies in living human cells reveals their dual role in mRNA decay and storage [J].