A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

被引：470
|
作者：
Polstein, Lauren R. [1 ,2 ,3 ]
Gersbach, Charles A. [1 ,2 ,3 ]
机构：
[1] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA
[2] Duke Univ, Ctr Genom & Computat Biol, Durham, NC USA
[3] Duke Univ, Med Ctr, Dept Orthopaed Surg, Durham, NC USA
来源：
NATURE CHEMICAL BIOLOGY | 2015年 / 11卷 / 03期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
TRANSCRIPTION FACTORS; SPATIOTEMPORAL CONTROL; PROTEIN INTERACTIONS; EXPRESSION; INDUCTION; CELLS;
D O I：
10.1038/nchembio.1753
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
引用
收藏
页码：198 / U179
页数：4
相关论文
共 50 条
  • [41] In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9
    Swiech, Lukasz
    Heidenreich, Matthias
    Banerjee, Abhishek
    Habib, Naomi
    Li, Yinqing
    Trombetta, John
    Sur, Mriganka
    Zhang, Feng
    NATURE BIOTECHNOLOGY, 2015, 33 (01) : 102 - U286
  • [42] Comparison of CRISPR-Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS
    Bruder, Mark R.
    Walji, Sadru-Dean
    Aucoin, Marc G.
    VIRUSES-BASEL, 2021, 13 (10):
  • [43] CRISPR-Cas9 assisted functional gene editing in the mushroom Ganoderma lucidum
    Wang, Ping-An
    Xiao, Han
    Zhong, Jian-Jiang
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 104 (04) : 1661 - 1671
  • [44] Editing the Melanocortin-4 Receptor Gene in Channel Catfish Using the CRISPR-Cas9 System
    Khalil, Karim
    Elaswad, Ahmed
    Abdelrahman, Hisham
    Michel, Maximillian
    Chen, Wenbiao
    Liu, Shikai
    Odin, Ramjie
    Ye, Zhi
    Drescher, David
    Vo, Khoi
    Bugg, William S.
    Qin, Guyu
    Yang, Yujia
    Backenstose, Nathan J. C.
    Liu, Zhanjiang
    Cone, Roger D.
    Dunham, Rex
    FISHES, 2023, 8 (02)
  • [45] CRISPR-Cas9 assisted gene disruption in the higher fungus Ganoderma species
    Qin, Hao
    Xiao, Han
    Zou, Gen
    Zhou, Zhihua
    Zhong, Jian-Jiang
    PROCESS BIOCHEMISTRY, 2017, 56 : 57 - 61
  • [46] Efficient CRISPR-Cas9 Gene Disruption System in Edible-Medicinal Mushroom Cordyceps militaris
    Chen, Bai-Xiong
    Wei, Tao
    Ye, Zhi-Wei
    Yun, Fan
    Kang, Lin-Zhi
    Tang, Hong-Biao
    Guo, Li-Qiong
    Lin, Jun-Fang
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [47] CRISPR-Cas9: A Potent Gene-editing Tool for the Treatment of Cancer
    Mishra, Gauri
    Srivastava, Kamakshi
    Rais, Juhi
    Dixit, Manish
    Singh, Vandana Kumari
    Mishra, Lokesh Chandra
    CURRENT MOLECULAR MEDICINE, 2024, 24 (02) : 191 - 204
  • [48] Spatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets
    Zhu, Haibao
    Zhang, Linlin
    Tong, Sheng
    Lee, Ciaran M.
    Deshmukh, Harshavardhan
    Bao, Gang
    NATURE BIOMEDICAL ENGINEERING, 2019, 3 (02) : 126 - 136
  • [49] Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
    Li, Jiao
    Song, Xin
    Xiong, Zhiqiang
    Wang, Guangqiang
    Xia, Yongjun
    Yang, Yijin
    Ai, Lianzhong
    MICROBIAL CELL FACTORIES, 2023, 22 (01)
  • [50] Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
    Das, Jutan
    Kumar, Sanjeev
    Mishra, Dwijesh Chandra
    Chaturvedi, Krishna Kumar
    Paul, Ranjit Kumar
    Kairi, Amit
    FRONTIERS IN GENETICS, 2023, 13
12345