A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

被引：470
|
作者：
Polstein, Lauren R. [1 ,2 ,3 ]
Gersbach, Charles A. [1 ,2 ,3 ]
机构：
[1] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA
[2] Duke Univ, Ctr Genom & Computat Biol, Durham, NC USA
[3] Duke Univ, Med Ctr, Dept Orthopaed Surg, Durham, NC USA
来源：
NATURE CHEMICAL BIOLOGY | 2015年 / 11卷 / 03期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
TRANSCRIPTION FACTORS; SPATIOTEMPORAL CONTROL; PROTEIN INTERACTIONS; EXPRESSION; INDUCTION; CELLS;
D O I：
10.1038/nchembio.1753
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
引用
收藏
页码：198 / U179
页数：4
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