Functional analysis of the aureothin iterative type I polyketide synthase

被引：51

作者：

He, J ^{[1
]}

Hertweck, C ^{[1
]}

机构：

[1] Hans Knoll Inst Nat Prod Res, D-07745 Jena, Germany

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 05期

关键词：

antibiotics; biosynthesis; natural products; polyketides; streptomyces;

D O I：

10.1002/cbic.200400333

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The modular-type polyketide synthase (PKS) that is involved in aureothin (aur) biosynthesis represents one of the first examples in which a single PKS module (AurA) is used in an iterative fashion. Here we report on the heterologous expression of an engineered AurAB fusion protein that unequivocally proves the iterative nature of AurA. In addition, point mutations reveal that aur PKS module 4 participates in polyketide biosynthesis despite its aberrant acyltronsferase domain.

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收藏

页码：908 / 912

页数：5

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