Nectin 4 Overexpression in Ovarian Cancer Tissues and Serum

被引：150

作者：

DeRycke, Melissa S. ^{[1
]}

Pambuccian, Stefan E. ^{[1
]}

Gilks, C. Blake ^{[3
]}

Kalloger, Steve E. ^{[3
]}

Ghidouche, Abderrezak ^{[4
]}

Lopez, Marc ^{[4
]}

Bliss, Robin L.

Geller, Melissa A. ^{[2
]}

Argenta, Peter A. ^{[2
]}

Harrington, Katherine M. ^{[1
]}

Skubitz, Amy P. N. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Obstet Gynecol & Womens Hlth, Minneapolis, MN 55455 USA

[3] British Columbia Canc Agcy, Cheryl Brown Ovarian Canc Outcomes Unit, Vancouver, BC V5Z 4E6, Canada

[4] Inst J Paoli I Calmettes, CRCM, INSERM, UMR 891, F-13009 Marseille, France

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2010年 / 134卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Nectin; 4; Ovarian cancer; Serum biomarker; Tissue microarray; Diagnosis; Detection; CELL-ADHESION; V-DOMAIN; IDENTIFICATION; CARCINOMA; ROLES; STATISTICS; EXPRESSION; INTERACTS; MARKER; FAMILY;

D O I：

10.1309/AJCPGXK0FR4MHIHB

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Early detection of ovarian cancer is difficult owing to the lack of specific and sensitive tests available. Previously, we found expression of nectin 4 to be increased in ovarian cancer compared with normal ovaries. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative RT-PCR validated the overexpress ion of nectin 4 messenger RNA in ovarian cancer compared with normal ovarian cell lines and tissues. Protein levels of nectin 4 were elevated in ovarian cancer cell lines and tissue compared with normal ovarian cell lines as demonstrated by Western immunoblotting, flow cytometry, and immunohistochemical staining of tissue microarray slides. Cleaved nectin 4 was detectable in a number of patient serum samples by enzyme-linked immunosorbent assay. In patients with benign gynecologic diseases with high serum CA 125 levels, nectin 4 was not detected in the majority of cases, suggesting that nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA 125.

引用

页码：835 / 845

页数：11

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