Foamy virus reverse transcriptase is expressed independently from the Gag protein

被引:88
作者
Enssle, J [1 ]
Jordan, I [1 ]
Mauer, B [1 ]
Rethwilm, A [1 ]
机构
[1] INST VIROL & IMMUNOBIOL, D-97078 WURZBURG, GERMANY
关键词
reverse transcriptase expression; ribosomal frameshifting; retrovirus; pararetrovirus;
D O I
10.1073/pnas.93.9.4137
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the foamy virus (FV) subgroup of retroviruses the pol genes are located in the +1 reading frame relative to the gag genes and possess potential ATG initiation codons in their 5' regions. This genome organization suggests either a +1 ribosomal frameshift to generate a Gag-Pol fusion protein, similar to all other retroviruses studied so far, or new initiation of Pol translation, as used by pararetroviruses, to express the Pol protein. By using a genetic approach we have ruled out the former possibility and provide evidence for the latter. Two down-mutations (M53 and M54) of the pol ATG codon were found to abolish replication and Pol protein expression of the human FV isolate. The introduction of a new ATG in mutation M55, 3' to the down-mutated ATG of mutation M53, restored replication competence, indicating that the pol ATG functions as a translational initiation codon. Two nonsense mutants (M56 and M57), which functionally separated gag and pol with respect to potential frame-shifting sites, were also replication-competent, providing further genetic evidence that FVs express the Pol protein independently from Gag. Our results show that during a particular step of the replication cycle, FVs differ fundamentally from all other retroviruses.
引用
收藏
页码:4137 / 4141
页数:5
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