Survey of a cDNA library from the turkey (Meleagris gallopavo)

被引：6

作者：

Chaves, LD ^{[1
]}

Rowe, JA ^{[1
]}

Reed, KM ^{[1
]}

机构：

[1] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

GENOME | 2005年 / 48卷 / 01期

关键词：

turkey; cDNA; expressed sequence tag; single nucleotide polymorphism;

D O I：

10.1139/G04-088

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genome characterization and analysis is an imperative step in identifying and selectively breeding for improved traits of agriculturally important species. Expressed sequence tags (ESTs) represent a transcribed portion of the genome and are an effective way to identify genes within a species. Downstream applications of EST projects include DNA microarray construction and interspecies comparisons. In this study, 694 ESTs were sequenced and analyzed from a library derived from a 24-day-old turkey embryo. The 437 unique sequences identified were divided into 76 assembled contigs and 361 singletons. The majority of significant comparative matches occurred between the turkey sequences and sequences reported from the chicken. Whole genome sequence from the chicken was used to identify potential exon-intron boundaries for selected turkey clones and intron-amplifying primers were developed for sequence analysis and single nucleotide polymorphism (SNP) discovery. Identified SNPs were genotyped for linkage analysis on two turkey reference populations. This study significantly increases the number of EST sequences available for the turkey.

引用

页码：12 / 17

页数：6

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