Luteogenesis in cyclic ewes: Echotextural, histological, and functional correlates

被引:30
作者
Duggavathi, R
Bartlewski, PM
Pierson, RA
Rawlings, NC
机构
[1] Univ Saskatchewan, WCVM, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B4, Canada
[2] Univ Saskatchewan, Coll Med, Dept Obstet Gynecol & Reprod Sci, Saskatoon, SK S7N 0W8, Canada
关键词
corpus luteum; corpus luteum function; ovary; ovulation; progesterone;
D O I
10.1095/biolreprod.103.016576
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
To date, it has not been possible to detect corpus luteum (CL) by ultrasonography, immediately following ovulation, in the ewe. Early CL detection is essential to be able to relate luteal outcome to the developmental pattern of the ovulated follicle and to confirm ovulation. Image analysis of the CL may be useful in providing a noninvasive picture of CL differentiation and function. The present study was designed to use high-resolution ultrasonography to monitor and to correlate the echotextural, histological, and functional attributes of the developing ovine CL from Days I to 3 after ovulation. Ten ewes underwent twice-daily transrectal ultrasonography and blood sampling from the day of synchronized estrus. Ewes were ovariectomized at 1224, 36-48, and 60-72 h after ovulation. Ovaries collected were scanned in a water bath before processing for histology. Ultrasonographic images of CL were analyzed for echotexture. Histological sections were analyzed for the percentage area of the CL occupied by blood clot or luteal tissue. Serum samples were analyzed for progesterone concentration. Numerical pixel value, heterogeneity, and percentage of the CL occupied by blood clot declined (P < 0.05) from 12-24 to 60-72 h after ovulation. Luteal area and serum progesterone concentration increased (P < 0.05) from 12-24 to 60-72 h. The results indicated that it was possible to visualize developing CL as early as 12-24 h after ovulation in the ewe. Echotexture of the CL was closely associated with its morphological and functional characteristics; image analysis holds promise for noninvasive monitoring of CL differentiation and growth.
引用
收藏
页码:634 / 639
页数:6
相关论文
共 37 条
[1]   BOVINE MODEL FOR STUDY OF OVARIAN FOLLICULAR DYNAMICS IN HUMANS [J].
ADAMS, GP ;
PIERSON, RA .
THERIOGENOLOGY, 1995, 43 (01) :113-120
[2]  
BAIRD DT, 1983, J REPROD FERTIL, V69, P343, DOI 10.1530/jrf.0.0690343
[3]   Ultrasound and endocrine evaluation of the ovarian response to PGF2α given at different stages of the luteal phase in ewes [J].
Barrett, DMW ;
Bartlewski, PM ;
Cook, SJ ;
Rawlings, NC .
THERIOGENOLOGY, 2002, 58 (07) :1409-1424
[4]   Ovarian responses in gonadotrophin-releasing hormone-treated anoestrous ewes: follicular and endocrine correlates with luteal outcome [J].
Bartlewski, PM ;
Beard, AP ;
Chapman, CL ;
Nelson, ML ;
Palmer, B ;
Aravindakshan, J ;
Cook, SJ ;
Rawlings, NC .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2001, 13 (2-3) :133-142
[5]   Ovarian function in ewes during the transition from breeding season to anoestrus [J].
Bartlewski, PM ;
Beard, AP ;
Rawlings, NC .
ANIMAL REPRODUCTION SCIENCE, 1999, 57 (1-2) :51-66
[6]   An ultrasonographic study of luteal function in breeds of sheep with different ovulation rates [J].
Bartlewski, PM ;
Beard, AP ;
Rawlings, NC .
THERIOGENOLOGY, 1999, 52 (01) :115-130
[7]  
BERNE RM, 1999, PRINCIPLES PHYSL, P181
[8]   Ultrasound studies of vascular and morphological changes in the human corpus luteum during the menstrual cycle [J].
Bourne, TH ;
Hagstrom, HG ;
Hahlin, M ;
Josefsson, B ;
Granberg, S ;
Hellberg, P ;
Hamberger, L ;
Collins, WP .
FERTILITY AND STERILITY, 1996, 65 (04) :753-758
[9]  
BRADEN TD, 1989, J REPROD FERTIL, V86, P525, DOI 10.1530/jrf.0.0860525
[10]  
Checura CM, 2002, THERIOGENOLOGY, V58, P533