Anticancer activity of biogenic nanosilver and its toxicity assessment on Artemia salina- evaluation of mortality, accumulation and elimination: An experimental report

The study was focused on assessment of anticancer activity and investigate the toxicity against Artemia salina adult and nauplii of biogenic silver nanoparticles (AgNPs) synthesized from in vitro derived Ceropegia thwaitesii leaf. These AgNPs were characterized using transmission electron micrograph (TEM) and Atomic force microscopy (AFM). TEM revealed the formation of mono dispersed with low polydispersity nanoparticles with size of 41.34 nm, and the presence of elemental silver was correlated with EDX. AFM topographical image conform the spherical nature of the AgNPs. These AgNPs exhibited a dose-dependent cytotoxicity against oral carcinoma (KB) cells and it was confirmed by MTT, Ao/EtBr, COMET assay and Rhodamine 123 staining. For toxicity study did not induce significant mortality against Artemia (adult and nauplii) at IC50 concentration 97.03 mg/ml at exposure time. The accumulation and elimination rate of nauplii was 7.5 and 0.85 mg/g and 14.5 and 1.06 mg/g at 24 and 108 h respectively, exposure at 200 mg/ml concentration. Whereas accumulation and elimination rate of adult was 9 and 17 mg/g and 1.15 and 1.86 mg/g at 24 and 108 h respectively, exposure at 200 mg/ml concentration. From these results A. salina test may expedite toxicity experiments and decrease the costs. (C) 2017 Elsevier Ltd. All rights reserved.