Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology

被引：42

作者：

Hamashima, Kiyofumi ^{[1
]}

Kimoto, Michiko ^{[1
]}

Hirao, Ichiro ^{[1
]}

机构：

[1] Inst Bioengn & Nanotechnol, 31 Biopolis Way,Nanos 07-01, Singapore 138669, Singapore

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2018年 / 46卷

基金：

新加坡国家研究基金会;

关键词：

SITE-SPECIFIC INCORPORATION; DNA APTAMER GENERATION; IN-VITRO SELECTION; RNA MOLECULES; SEMISYNTHETIC ORGANISM; HYDROPHOBIC BASE; PHARMACEUTICAL APPLICATIONS; ENZYMATIC INCORPORATION; EXPONENTIAL ENRICHMENT; LABORATORY EVOLUTION;

D O I：

10.1016/j.cbpa.2018.07.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Artificial extra base pairs (unnatural base pairs, UBPs) expand the genetic alphabet of DNA, thus broadening entire biological systems in the central dogma. UBPs function as third base pairs in replication, transcription, and/or translation, and have created a new research area, synthetic xenobiology, providing genetic engineering tools to generate novel DNAs, RNAs, and proteins with increased functionalities. Several UBPs have been developed and applied to PCR technology, DNA aptamer generation, and semi-synthetic organism creation. Among them, we developed a series of UBPs and demonstrated unique quantitative PCR and high-affinity DNA aptamer generation methods.

引用

页码：108 / 114

页数：7

共 48 条

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[48] Toward a designed genetic system with biochemical function: polymerase synthesis of single and multiple size-expanded DNA base pairs
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