Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo

被引：15

作者：

Wang, Jiwen ^{[1
,2
]}

Yao, Yanli ^{[2
]}

Ming, Yue ^{[3
]}

Shen, Sheng ^{[1
]}

Wu, Nan ^{[4
,5
]}

Liu, Jiaqi ^{[6
]}

Liu, Han ^{[1
]}

Suo, Tao ^{[1
]}

Pan, Hongtao ^{[1
]}

Zhang, Dexiang ^{[7
]}

Ding, Kan ^{[2
]}

Liu, Houbao ^{[1
]}

机构：

[1] Fudan Univ, Gen Surg Inst, Zhongshan Hosp, Dept Gen Surg, Shanghai 200030, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Mat Med, Glycobiol & Glycochem Lab, Shanghai 201203, Peoples R China

[3] Chinese Acad Med Sci, Canc Hosp, Peking Union Med Coll, PET CT Ctr, Beijing 100021, Peoples R China

[4] Beijing Key Lab Genet Res Skeletal Deform, Beijing, Peoples R China

[5] Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Orthopaed Surg, Beijing 100730, Peoples R China

[6] Chinese Acad Med Sci, Canc Hosp, Dept Breast Surg Oncol, Beijing 100021, Peoples R China

[7] Fudan Univ, Peoples Hosp Shanghai 5, Dept Gen Surg, Shanghai 200240, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

CANCER; TARGET; SUPPRESSOR; EXPRESSION; PROTEIN; LACKING;

D O I：

10.1038/srep28833

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Stathmin 1 (STMN1) is an important cytosolic protein associated with microtubule stability that was reported to be involved in tumorigenesis. Up to our knowledge, its role in gallbladder carcinoma has not been analyzed. In this study, we found that STMN1 was significantly highly expressed in GBC by immunohistochemistry (IHC). Further research demonstrated that silencing of STMN1 inhibited cell growth in vitro. Moreover, knockdown of STMN1 induced apoptosis and delayed G2/M phase transformation in GBC cells. Our data support a rationale for further studies that the silencing of STMN1 may regulate the activity of p38 MAPK kinase and p53/p21 signal pathway. Besides, xenografted gallbladder carcinoma cells growth were significantly impaired after STMN1 was silenced in vivo. These results suggested that STMN1 played an important role in cell proliferation and migration. This provided a potential clue for investigating the therapeutic target in GBC.

引用

页数：8

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