NtRLK5, a novel RLK-like protein kinase from Nitotiana tobacum, positively regulates drought tolerance in transgenic Arabidopsis

被引：4

作者：

Xu, Guoyun ^{[1
]}

Li, Mingjuan ^{[2
]}

Zhang, Hui ^{[1
]}

Chen, Qiansi ^{[1
]}

Jin, Lifeng ^{[1
]}

Zheng, Qingxia ^{[1
]}

Liu, Pingping ^{[1
]}

Cao, Peijian ^{[1
]}

Chen, Xia ^{[1
]}

Zhai, Niu ^{[1
]}

Zhou, Huina ^{[1
]}

机构：

[1] China Natl Tobacco Corp, Zhengzhou Tobacco Res Inst, Zhengzhou, Henan, Peoples R China

[2] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 03期

关键词：

Nitotiana tobacum; Drought tolerance; RLKs; Arabidopsis; NtRLK5; RECEPTOR-LIKE KINASES; SALT TOLERANCE; LIPID-PEROXIDATION; RESPONSIVE GENES; SALINITY STRESS; YIELD PENALTY; EXPRESSION; THALIANA; RICE; OVEREXPRESSION;

D O I：

10.1016/j.bbrc.2018.07.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Receptor-like protein kinase (RLKs) plays pivotal roles in plant growth and development as well as stress responses. However, little is known about the function of RLKs in Nitotiana tobacum. In the present study, we present data on NtRLK5, a novel RLK-like gene isolated from Hongda (Nitotiana tobacum L). Expression profile analysis revealed that NtRLK5 was strongly induced by drought and salt stresses. Transient expression of NtRLK5-GFP fusion protein in protoplast showed that NtRLK5 was localized to plasma membrane. Overexpression of NtRLK5 conferred enhanced drought tolerance in transgenic Arabidopsis plants, which was attributed to not only the lower malondialdehyde (MDA) and H2O2 contents, but also the higher antioxidant enzymes activities. Moreover, the expression of several anti-oxidation-and stress-related genes was also significantly up-regulated in NtRLK5 transgenic plants under drought condition. Taken together, the results suggest that NtRLK5 functions as a positive regulator in drought tolerance. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：1235 / 1240

页数：6

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