Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

被引：122

作者：

Mizuno, Yuta

Chang, Hsi

Umeda, Katsutsugu

Niwa, Akira

Iwasa, Toru

Awaya, Tomonari

Fukada, So-ichiro ^{[4
]}

Yamamoto, Hiroshi ^{[4
]}

Yamanaka, Shinya ^{[2
,3
,5
]}

Nakahata, Tatsutoshi ^{[3
]}

Heike, Toshio ^{[1
]}

机构：

[1] Kyoto Univ, Dept Pediat, Grad Sch Med, Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Dept Stem Cell Biol, Inst Frontier Med Sci, Kyoto 6068507, Japan

[3] Kyoto Univ, Ctr iPS Cell Res & Applicat, Inst Integrated Cell Mat Sci, Kyoto 6068507, Japan

[4] Osaka Univ, Dept Immunol, Grad Sch Pharmaceut Sci, Osaka, Japan

[5] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama, Japan

来源：

FASEB JOURNAL | 2010年 / 24卷 / 07期

关键词：

Duchenne muscular dystrophy; Pax7; long-term engraftment; no teratoma formation; high engraftment efficiency; BONE-MARROW; DYSTROPHIN EXPRESSION; SATELLITE CELLS; MOUSE; REGENERATION; FIBROBLASTS; PROGENITORS; CULTURE; MDX;

D O I：

10.1096/fj.09-137174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6(+) cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.-Mizuno, Y., Chang, H., Umeda, K., Niwa, A., Iwasa, T., Awaya, T., Fukada, S., Yamamoto, H., Yamanaka, S., Nakahata, T., Heike, T. Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB J. 24, 2245-2253 (2010). www.fasebj.org

引用

页码：2245 / 2253

页数：9