HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1

被引：67
|
作者：
Figaro, Sabine [1 ]
Scrima, Nathalie [1 ]
Buckingham, Richard H. [1 ]
Heurgue-Hamard, Valerie [1 ]
机构：
[1] IBPC, CNRS, UPR 9073, F-75005 Paris, France
来源：
FEBS LETTERS | 2008年 / 582卷 / 16期
关键词：
HemK2; methyltransferase; eRF1; N5-methylglutamine; hTrm112; human chromosome 21;
D O I：
10.1016/j.febslet.2008.05.045
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ubiquitous tripeptide Gly-Gly-Gln in class 1 polypeptide release factors triggers polypeptide release on ribosomes. The Gln residue in both bacterial and yeast release factors is N5-methylated, despite their distinct evolutionary origin. Methylation of eRF1 in yeast is performed by the heterodimeric methyltransferase ( MTase) Mtq2p/Trm112p, and requires eRF3 and GTP. Homologues of yeast Mtq2p and Trm112p are found in man, annotated as an N6-DNA-methyltransferase and of unknown function. Here we show that the human proteins methylate human and yeast eRF1. eRF3. GTP in vitro, and that the MTase catalytic subunit can complement the growth defect of yeast strains deleted for mtq2.
引用
收藏
页码：2352 / 2356
页数：5
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