In vivo assessment of polydatin, a natural polyphenol compound, on arsenic-induced free radical overproduction, gene expression, and genotoxicity

被引：29

作者：

Arslan-Acaroz, Damla ^{[1
]}

Zemheri, Fahriye ^{[2
]}

Demirel, Hasan Huseyin ^{[3
]}

Kucukkurt, Ismail ^{[1
]}

Ince, Sinan ^{[4
]}

Eryavuz, Abdullah ^{[5
]}

机构：

[1] Afyon Kocatepe Univ, Fac Vet Med, Dept Biochem, TR-03200 Afyon, Turkey

[2] Bartin Univ, Fac Art & Sci, Dept Mol Biol & Genet, TR-74100 Bartin, Turkey

[3] Afyon Kocatepe Univ, Bayat Vocat Sch, Bayat, Afyonkarahisar, Turkey

[4] Afyon Kocatepe Univ, Fac Vet Med, Dept Pharmacol & Toxicol, TR-03200 Afyon, Turkey

[5] Afyon Kocatepe Univ, Fac Vet Med, Dept Physiol, TR-03200 Afyon, Turkey

来源：

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2018年 / 25卷 / 03期

关键词：

Polydatin; Arsenic; DNA damage; Gene expression; Oxidative stress; Rat; INDUCED OXIDATIVE STRESS; INDUCED LIPID-PEROXIDATION; DNA-DAMAGE; CELLS; ACID; ACTIVATION; RESPONSES; BORON; ASSAY;

D O I：

10.1007/s11356-017-0391-6

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Arsenic (As) is a well-known contaminant of global groundwater. Its exposure causes several hazardous effects on animals and human via oxidative stress. The present study examined the effect of polydatin (PD) on free radical overproduction in rats exposed to As. Thirty-five male rats randomly allocated into five equal groups. To the control group, physiological saline was given orally and to the second group only 100 mg/L As was given by drinking water for 60 days. The other groups were treated with As (100 mg/L) and PD orally at 50, 100, and 200 mg/kg/day, respectively. Treatment with As enhanced malondialdehyde level but decreased glutathione level in blood, liver, kidney, brain, lung, and heart of rats. Also, As decreased superoxide dismutase and catalase activities of erythrocyte, liver, kidney, brain, lung, and heart in rats. Furthermore, As treatment gave rise to increased DNA damage and gene expressions of interleukin 1 beta (IL-1 beta), nuclear factor kappa beta (NF kappa B), p53, and tumor necrosis factor-alpha (TNF-alpha) in the lung, brain, kidney, and liver. However, treatment of PD ameliorated As-exposed lipid peroxidation, antioxidant enzymes activities, DNA damage, gene expressions, and histopathological changes in tissues. In conclusion, PD has a dose-dependent protective effect on lipid peroxidation and antioxidant defense mechanism in rats against As exposure.

引用

页码：2614 / 2622

页数：9

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