Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester

被引：6
|
作者：
Juzenas, P [1 ]
Sorensen, R [1 ]
Iani, V [1 ]
Moan, J [1 ]
机构：
[1] Norwegian Radium Hosp, Inst Canc Res, Dept Biophys, N-0310 Oslo, Norway
来源：
PHOTOHEMOTHERAPY OF CANCER AND OTHER DISEASES, PROCEEDINGS | 1999年 / 3563卷
关键词：
5-aminolevulinic acid (ALA); 5-aminolevulinic acid methyl ester (ALA-Me); protoporphyrin IX (PpIX); topical application;
D O I：
10.1117/12.339134
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The clearance of protoporphyrin IX (PpIX) from the skin of hairless BALB/c mice after topical application of 5-aminolevulinic acid (ALA) and its methyl ester (ALA-Me) was investigated. Creams containing 2 or 20% of ALA or ALA-Me were topically applied on spots of approximately 1 cm(2) for 12 hours. The PpIX fluorescence was detected by the means of a Perkin Elmer LS50B luminescence spectrometer equipped with a fibre-optic probe. The emission spectrum was identical with that of cell-bound PpIX. After 12 hours application of ALA and ALA-Me similar amounts of PpIX were found. After cream removal the ALA-induced PpIX fluorescence decayed with a half-life of about 20 hours (20% ALA cream). The ALA-Me-induced PpIX was faster cleared from the skin than ALA-induced PpIX, and had a half-life of about 7 hours (20% ALA-Me cream).
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页码：161 / 166
页数：6
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