Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

被引：2
|
作者：
Ying, Zhongfu [1 ,2 ]
Liu, Zihuang [1 ,2 ]
Xiang, Ge [1 ,2 ]
Xin, Yanmin [1 ,2 ]
Wang, Junwei [1 ,2 ]
Liu, Xingguo [1 ,2 ,3 ]
机构：
[1] Guangzhou Med Univ, Guangzhou Inst Biomed & Hlth, Chinese Acad Sci, Joint Sch Life Sci,CAS Key Lab Regenerat Biol, Guangzhou 510530, Peoples R China
[2] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Stem Cell & Regenerat, Guangzhou Inst Biomed & Hlth,Bioland Lab,Guangzhou, Guangzhou 510530, Peoples R China
[3] Chinese Acad Sci, Hong Kong Inst Sci & Innovat, Ctr Regenerat Med & Hlth, Hong Kong, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
关键词：
Cell Biology; Cell-based Assays; Flow Cytometry/Mass Cytometry; Metabolism; Microscopy; Stem Cells;
D O I：
10.1016/j.xpro.2021.100568
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high and low mPTP opening somatic cells by calcein fluorescence and reprogram these sorted cells to iPSCs. These protocols are also suitable for imaging mPTP opening and uncovering the mechanisms of mPTP function in other cell fate conversions. For complete details on the use and execution of this protocol, please refer to Ying et al. (2018).
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页数：12
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