Electrostatic complex of didodecyldimethylammonium and DNA: Langmuir monolayer, Langmuir-Blodgett film and dye recognition at air/water interface

yDNA-didodecyldimethylammonium (DNA-DDDA) electrostatic complex was prepared and characterized through Fourier transformation infrared (FT-IR), H-1 NMR and circular dichroism (CD) spectroscopy. When the dye molecule aqueous solutions were used as the subphase, the interaction between three dye molecules, acridine orange (AO), ethidium bromide (EB) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and the complex at air/solution interface were investigated through the surface pressure-area (pi-A) isotherms, Brewster angle microscopy and UV-Vis spectroscopy, respectively. Our investigation indicates that the interaction capabilities of the three dyes to DNA-DDDA complex are different and present an order of TMPyP > AO > EB. For the interaction forms, we believe that TMPyP intercalates into the double helix of DNA, and AO adsorbs onto the surface of the DNA. As for EB, the measured signal is too weak to give a definite interaction form in the present experiment. (C) 2003 Elsevier B.V. All rights reserved.