Electrostatic complex of didodecyldimethylammonium and DNA: Langmuir monolayer, Langmuir-Blodgett film and dye recognition at air/water interface

被引：7

作者：

Hou, XL ^{[1
]}

Sun, L ^{[1
]}

Xu, M ^{[1
]}

Wu, LX ^{[1
]}

Shen, JC ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Minist Educ, Key Lab Supramol Struct & Mat, Changchun 130023, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2004年 / 33卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

electrostatic complex; DNA; dye; recognition; air/water interface;

D O I：

10.1016/j.colsurfb.2003.10.001

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

yDNA-didodecyldimethylammonium (DNA-DDDA) electrostatic complex was prepared and characterized through Fourier transformation infrared (FT-IR), H-1 NMR and circular dichroism (CD) spectroscopy. When the dye molecule aqueous solutions were used as the subphase, the interaction between three dye molecules, acridine orange (AO), ethidium bromide (EB) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and the complex at air/solution interface were investigated through the surface pressure-area (pi-A) isotherms, Brewster angle microscopy and UV-Vis spectroscopy, respectively. Our investigation indicates that the interaction capabilities of the three dyes to DNA-DDDA complex are different and present an order of TMPyP > AO > EB. For the interaction forms, we believe that TMPyP intercalates into the double helix of DNA, and AO adsorbs onto the surface of the DNA. As for EB, the measured signal is too weak to give a definite interaction form in the present experiment. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：157 / 163

页数：7

共 24 条

[1] Stacking and mobility of cationic amphiphiles in the complex with DNA and DNA transfection activity of the complex [J].