Slow controlled-rate freezing of human in vitro matured oocytes: effects on maturation rate and kinetics and parthenogenetic activation

被引：13

作者：

Versieren, Karen ^{[1
]}

Heindryckx, Bjorn ^{[1
]}

O'Leary, Thomas ^{[1
]}

De Croo, Ilse ^{[1
]}

Van den Abbeel, Etienne ^{[1
]}

Gerris, Jan ^{[1
]}

De Sutter, Petra ^{[1
]}

机构：

[1] Ghent Univ Hosp, Dept Reprod Med, B-9000 Ghent, Belgium

来源：

FERTILITY AND STERILITY | 2011年 / 96卷 / 03期

关键词：

Slow controlled-rate freezing; human oocytes; in vitro maturation; artificial activation; METAPHASE-II OOCYTES; EMBRYO DEVELOPMENT; GERMINAL VESICLE; IMMATURE OOCYTES; CRYOPRESERVATION; FERTILIZATION; VITRIFICATION; FRESH; CONFIGURATIONS; CHROMOSOME;

D O I：

10.1016/j.fertnstert.2011.06.060

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To create a pool of frozen donated human oocytes and find the optimal stage for slow controlled-rate freezing of human in vitro matured oocytes. Design: Oocytes at different developmental stages of maturation (germinal vesicle, metaphase I, or metaphase II) and oocytes that failed to fertilize after IVF or intracytoplasmic sperm injection (ICSI) were frozen using a slow controlled-rate freezing protocol. Frozen/thawed oocytes were artificially activated to verify activation potential and compared with oocytes that were not frozen. Setting: University hospital-based fertility center. Patient(s): Stimulated patients undergoing IVF/ICSI treatment donated oocytes left over during their infertility treatment. Intervention(s): Human oocytes were frozen at different stages of maturation. Fresh and frozen/thawed oocytes were activated by electrical pulses followed by incubation in 6-dimethylaminopurine. Main Outcome Measure(s): Survival rate, maturation rate and kinetics, and activation potential. Result(s): Human oocytes at all developmental stages have high survival rates after slow controlled-rate freezing. Frozen/thawed germinal vesicle oocytes showed decreased and delayed maturation after thawing. Activation potential was not affected. Conclusion(s): A pool of donated human oocytes could be established using slow controlled-rate freezing. Immature oocytes should be frozen after in vitro maturation. (Fertil Steril (R) 2011;96:624-8. (C)2011 by American Society for Reproductive Medicine.)

引用

页码：624 / 628

页数：5