Electropermeabilization of cell membranes

被引:169
作者
Teissié, J [1 ]
Eynard, N [1 ]
Gabriel, B [1 ]
Rols, MP [1 ]
机构
[1] CNRS, UPR 9062, IPBS, F-31062 Toulouse, France
关键词
electrotransformation; electroinsertion; electrofusion; electrochemotherapy; drug delivery; gene transfer; oxidative stress;
D O I
10.1016/S0169-409X(98)00060-X
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
A position dependent modulation of the membrane potential difference is induced when an electric field is applied to a cell, When cells are submitted to short lived electric field pulses with an overcritical intensity, a local membrane alteration is induced, which may reseal. Its molecular definition remains unknown. A free exchange of hydrophilic molecules takes place across the membrane. A leakage of cytosolic metabolites is present. However, a loading of polar drugs into the cytoplasm is obtained, A short description of the processes affecting the cell membrane organization is given, Lipids appear as the primary target of the field effect as in the case of liposomes. Nevertheless membrane proteins appear to be affected by a direct or by a back effect. The permeabilized state is long lived. The cell metabolism plays indeed a critical role in the recovery. The cell viability can be nevertheless preserved. (C) 1999 Elsevier Science B.V, All rights reserved.
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页码:3 / 19
页数:17
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