3D printed carboxymethyl cellulose scaffolds for autologous growth factors delivery in wound healing

被引：95

作者：

Diaz-Gomez, Luis ^{[1
,2
]}

Gonzalez-Prada, Iago ^{[1
,2
]}

Millan, Rosendo ^{[3
]}

Da Silva-Candal, Andres ^{[4
,5
]}

Bugallo-Casal, Ana ^{[4
]}

Campos, Francisco ^{[4
]}

Concheiro, Angel ^{[1
,2
]}

Alvarez-Lorenzo, Carmen ^{[1
,2
]}

机构：

[1] Univ Santiago de Compostela, Fac Farm, Dept Farmacol Farm & Tecnol Farmaceut, I D Farma GI 1645, Santiago De Compostela 15782, Spain

[2] Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Santiago De Compostela 15782, Spain

[3] Univ Santiago de Compostela, Ctr Biomed Expt USC CEBEGA, Santiago De Compostela 15706, Spain

[4] Univ Santiago de Compostela, Clin Univ Hosp, Hlth Res Inst Santiago de Compostela IDIS, Clin Neurosci Res Lab, Santiago De Compostela, Spain

[5] Univ Hosp Complex A Coruna, Neurol Serv, Neurovasc Dis Lab, Biomed Res Inst INIBIC, La Coruna 15706, Spain

来源：

CARBOHYDRATE POLYMERS | 2022年 / 278卷

关键词：

Carboxymethyl cellulose; 3D printing; Rheological properties; microCT; Platelet rich plasma; Wound healing; Cell mobility; Angiogenesis; PLATELET-RICH PLASMA; DRUG-DELIVERY; CROSS-LINKING; HYDROGELS; DERIVATIVES; MANAGEMENT; RELEASE; MATRIX; FILMS; CARE;

D O I：

10.1016/j.carbpol.2021.118924

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This work aims to use carboxymethyl cellulose (CMC) as main structural and functional component of 3D printed scaffolds for healing of diabetic wounds. Differently from previous inks involving small contents in CMC, herein sterile (steam-heated) concentrated CMC solely dispersions (10-20%w/v) were screened regarding printability and fidelity properties. CMC (15%w/v)-citric acid inks showed excellent self-healing rheological properties and stability during storage. CMC scaffolds loaded with platelet rich plasma (PRP) sustained the release of relevant growth factors. CMC scaffolds both with and without PRP promoted angiogenesis in ovo, stem cell migration in vitro, and wound healing in a diabetic model in vivo. Transparent CMC scaffolds allowed direct monitoring of bilateral full-thickness wounds created in rat dorsum. CMC scaffolds facilitated re-epithelialization, granulation, and angiogenesis in full-thickness skin defects, and the performance was improved when combined with PRP. Overall, CMC is pointed out as outstanding component of active dressings for diabetic wounds.

引用

页数：12

共 66 条

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