Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by 1H Liquid-State NMR Spectroscopy and Biochemical Methods

被引：4

作者：

Larsen, Flemming H. ^{[1
]}

Essen-Gustavsson, Birgitta ^{[3
]}

Jensen-Waern, Marianne ^{[3
]}

Lametsch, Rene ^{[1
]}

Karlsson, Anders H. ^{[1
]}

Lindahl, Gunilla ^{[1
,2
]}

机构：

[1] Univ Copenhagen, Dept Food Sci, DK-1958 Frederiksberg C, Denmark

[2] Swedish Univ Agr Sci, Dept Food Sci, SE-75007 Uppsala, Sweden

[3] Swedish Univ Agr Sci, Dept Clin Sci, SE-75007 Uppsala, Sweden

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 22期

基金：

瑞典研究理事会;

关键词：

glycogen; H-1 nuclear magnetic resonance (H-1 NMR); enzymatic analysis; pork; RN-gene; PRKAG3; mutation; PORCINE MUSCLE; MEAT QUALITY; EVOLUTION; HYDRATION; SPECTRA; IMPACT; TIME; PIG;

D O I：

10.1021/jf201822p

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

引用

页码：11895 / 11902

页数：8

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