Determination of fluoxetine in plasma by gas chromatography-mass spectrometry using stir bar sorptive extraction

被引：45

作者：

Fernandes, Christian ^{[1
]}

Van Hoeck, Els ^{[2
]}

Sandra, Pat ^{[2
]}

Lancas, Fernando M. ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Chem Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil

[2] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 614卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

stir bar sorptive extraction; fluoxetine; thermal desorption; in situ derivatization; plasma;

D O I：

10.1016/j.aca.2008.03.036

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article presents a method employing stir bar sorptive extraction (SBSE) with in situ derivatization, in combination with either thermal or liquid desorption on-line coupled to gas chromatography-mass spectrometry for the analysis of fluoxetine in plasma samples. Ethyl chloroformate was employed as derivatizing agent producing symmetrical peaks. Parameters such as solvent polarity, time for analyte desorption, and extraction time, were evaluated. During the validation process, the developed method presented specificity, linearity (R-2 > 0.99), precision (R.S.D. < 15%), and limits of quantification (LOQ) of 30 and 1.37 pg mL(-1), when liquid and thermal desorption were employed, respectively. This simple and highly sensitive method showed to be adequate for the measurement-of fluoxetine in typical and trace concentration levels. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：201 / 207

页数：7

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