Formation of aminosilane-functionalized mica for atomic force microscopy imaging of DNA

被引:65
作者
Crampton, N
Bonass, WA
Kirkham, J
Thomson, NH [1 ]
机构
[1] Univ Leeds, Sch Phys & Astron, Leeds LS2 9JT, W Yorkshire, England
[2] Univ Leeds, Dept Oral Biol, Leeds LS2 9LU, W Yorkshire, England
关键词
D O I
10.1021/la050972q
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Factors affecting the functionalization of mica with aminosilanes, in particular, aminopropyltriethoxysilane (APTES-mica), formed from the vapor phase have been systematically studied. The relative humidity (RH) during vapor deposition has been varied, and postdeposition treatment through baking has been used, as well as the comparison of mono and trifunctionality, to investigate how optimal surfaces for AFM imaging of DNA are formed. It is found that the stability of the APTES layers is a consequence of lateral polymerization and not covalent attachment to the mica substrate. At low RH (< 25%), DNA adopts an open, well-resolved conformation, whereas at > 25% RH, DNA surface-induced condensation occurs. Contact mode AFM scratching experiments show that two main structures of the silane layer exist at different humidity: a monolayer exists at RH < 25%, and a bilayer structure exists at RH > 25%. Finally, structural changes that these two layer types undergo after baking at 150 degrees C were investigated by AFM and X-ray photoelectron spectroscopy (XPS), and these now prevented DNA from binding to the APTES-mica, except in the presence of Mg(II) ions.
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收藏
页码:7884 / 7891
页数:8
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