Synaptic vesicles studied by dynamic light scattering

被引：12

作者：

Castorph, S. ^{[1
]}

Henriques, S. Schwarz ^{[1
]}

Holt, M. ^{[2
]}

Riedel, D. ^{[3
]}

Jahn, R. ^{[2
]}

Salditt, T. ^{[1
]}

机构：

[1] Univ Gottingen, Inst Rontgenphys, Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany

[3] Max Planck Inst Biophys Chem, Elect Microscopy Grp, D-37077 Gottingen, Germany

来源：

EUROPEAN PHYSICAL JOURNAL E | 2011年 / 34卷 / 06期

关键词：

FIELD-FLOW FRACTIONATION; FREDHOLM INTEGRAL-EQUATIONS; SIZE DISTRIBUTION; MEMBRANE; FUSION; LIPOSOMES; AGGREGATION;

D O I：

10.1140/epje/i2011-11063-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The size polydispersity distribution of synaptic vesicles (SVs) is characterized under quasi-physiological conditions by dynamic light scattering (DLS). Highly purified fractions of SVs obtained from rat brain still contain a small amount of larger contaminant structures, which can be quantified by DLS and further reduced by asymmetric-flow field-flow (AFFF) fractionation. The intensity autocorrelation functions g(2)(tau) recorded from these samples are analyzed by a constrained regularization method as well as by an alternative direct modeling approach. The results are in quantitative agreement with the polydispersity obtained from cryogenic electron microscopy of vitrified SVs. Next, different vesicle fusion assays based on samples composed of SVs and small unilamellar proteoliposomes with the fusion proteins syntaxin 1 and SNAP-25A are characterized by DLS. The size increase of the proteoliposomes due to SNARE-dependent fusion with SVs is quantified by DLS under quasi-physiological conditions.

引用

页码：1 / 11

页数：11

共 40 条

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