Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads

被引：115

作者：

Roy, I ^{[1
]}

Gupta, MN ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2004年 / 34卷 / 01期

关键词：

calcium alginate beads; enzyme immobilization; glucoamylase; pullulanase; starch hydrolysis;

D O I：

10.1016/j.enzmictec.2003.07.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hydrolysis of starch was carried out by a blend of individually entrapped glucoamylase and pullulanase (in calcium alginate beads) in the ratio of 3:2 (activity wise). The individually entrapped enzymes show enhanced thermostability at 55degreesC. The mixture of the immobilized enzymes could be used both in packed as well as fluidized bed formats to obtain DE values of 95 and 96, respectively. It is shown that this is more efficient than using glucoamylase alone or adding the two enzymes in a sequential fashion, since in the latter approach, the products of the first enzyme inhibit the second enzyme. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：26 / 32

页数：7

共 34 条

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